The HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtiter plate coated with HIV antigen. If HIV antibodies are present in the blood they will bind to the antigens on the plate. This binding is detected with an enzyme-linked secondary antibody that causes a colour change upon addition of substrate.In this experiment your students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.Group Size: for 10 lab groupsStorage: RefrigeratorTime Required: 60 minutesThe kit includes:Full instructions10X ELISA wash bufferELISA dilution bufferAntigen (lypholized)Primary antibody (lypholized)Secondary antibody (lypholized)ABTS (lypholized)ABTS reaction bufferMicrotitre platesTransfer pipettesSnap-top microcentrifuge tubes15ml conical tubesAdditional required materials (not included with the kit):Distilled waterBeakersDisposable laboratory glovesAutomatic Micropipette & tips (recommended)Incubator (B8A64397)