In this Transformation of E-Coli Kit students will explore the biological process of bacterial transformation using E-Coli and green fluorescent protein plasmid DNA.At the end of the activity students will have experience observing and analysing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.Group Size: for 10 lab groupsStorage: some components require refrigerator and freezer storage upon receiptTime Required: Set-up - 50 minutes Incubation - overnight Transformation - 15 minutesThe kit includes:Full instructionsBactoBeads™ E.coli GFP HostSupercoiled pFluoroGreen™ plasmid DNAAmpicillinIPTGCalcium ChlorideGrowth AdditiveReadyPour™ Luria Broth Agar (sterile)Luria Broth Medium for Recovery (sterile)Petri Plates (small & large)Plastic microtipped transfer pipettesWrapped 10ml pipette (sterile)Toothpicks (sterile)Inoculating loops (sterile)Microcentrifuge tubesAdditional required equipment & materials (not supplied with the kit):Automatic micropipette (5-50µl)2 x Water Bath (B8R06048)Thermometer (B8R05775)Incubator (B8A64397)Pipette Filler (B8A78062)IceMarker PensBunsen BurnerMicrowave or Hot PlateHot glovesLong wave UV light